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. Author manuscript; available in PMC: 2010 Feb 12.
Published in final edited form as: Neuron. 2009 Feb 12;61(3):425–438. doi: 10.1016/j.neuron.2008.12.015

Figure 1. Association of CaMKIIα with D3Rs.

Figure 1

(A) GST-fusion proteins derived from rat D3Rs with 7 transmembrane (TM) domains. (B) Coomassie staining of a gel resolved from pull-down assays with rat accumbal lysates. An open arrow indicates a protein (~50 kDa) pulled down by GST-D3RIL3. (C) GST-D3RIL3 precipitated CaMKIIα from rat accumbal lysates. (D) GST-fusion proteins derived from rat CaMKIIα. (E and F) GST-CaMKIIα and GST-CaMKIIαCD precipitated D3Rs (E), but not D1Rs (F), from rat accumbal lysates. (G) GST-fusion proteins derived from D3RIL3. (H–J) Results from in vitro binding assays with GST-fusion proteins and purified CaMKIIα (H and I) or D3RIL3 (J). Precipitated proteins from pull-down assays (C, E, and F) and proteins bound to CaMKIIα or D3RIL3 from in vitro binding assays (H–J) were visualized with immunoblots (IB) using the specific antibodies as indicated.