Figure 2.
Tyrosine phosphorylation in BMM after selective ligation of FcγRI or FCγRII/III. (A) BMM were stimulated with purified mouse IgG2a (UPC10 myeloma protein) followed by F(ab′)2 of goat anti-mouse IgG. Cells were harvested at 0 or 10 min, and lysates were immunoprecipitated (IP) with the indicated antibodies. Western blots were probed initially with 4G10, then stripped and reprobed with the immunoprecipitating antibody. (B) BMM were stimulated with F(ab′)2 of 2.4G2 followed by F(ab′)2 of goat anti-rat IgG. Immunoprecipitation and Western blots were as described in A. Data were replicated in three separate experiments using different mice.