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. 2009 Feb 3;106(8):2571–2576. doi: 10.1073/pnas.0813180106

Fig. 2.

Fig. 2.

Representative FRET time trajectories of L1 stalk dynamics. Traces show fluorescence intensities observed for Cy3 (green) on ribosomal protein L33 and Cy5 (red) on ribosomal protein L1 and the calculated FRET trace in blue. The fitted HaMMy curves are superimposed on the FRET trajectories in black. Fluorescence intensities are only shown for the complex in A. Complexes were formed from labeled 70S ribosomes containing tRNATyr in the P site and N-Ac-Phe-tRNAPhe in the A site (A), tRNAfMet in the P site and N-Ac-Phe-tRNAPhe in the A site (B), or tRNAfMet in the P site with a vacant A site (C).