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. 2009 Feb 3;106(8):2571–2576. doi: 10.1073/pnas.0813180106

Fig. 3.

Fig. 3.

FRET histograms for pretranslocation and posttranslocation complexes. (A–C) L1(Cy5)/L33(Cy3)-labeled ribosomes programmed with m301 mRNA, containing (A) tRNATyr in the P site and N-Ac-Phe-tRNAPhe in the A site; (B) as in A with 300 nM EF-G and 250 μM GTP; and (C) as in B with 100 nM tRNATyr added to the imaging buffer. (D–F) FRET histograms for L1(Cy5)/L33(Cy3)-labeled ribosomes programmed with m291 mRNA and containing (D) N-Ac-Phe-tRNAPhe in the P site; (E) N-Ac-Phe-tRNAPhe in the P site with 144 nM tRNAfMet added to the imaging buffer; (F) tRNAPhe in the P site after deacylation of the complex in D with puromycin. (G and H) FRET histograms for S6(Cy5)/L9(Cy3)-labeled ribosomes containing (G) N-Ac-Phe-tRNAPhe in the P site; and (H) N-Ac-Phe-tRNAPhe in the P site with 200 nM tRNAfMet added to the imaging buffer.