Fig. 1.

The diagram structures of 2 proteins studied in this paper. The locations of active sites are shown by van der Waals surfaces for the substrates. Positions of the substitutions are shown by gray circles corresponding to the backbone CA atoms. (A) Acylphosphatase (AcPh): comparison of the structures of bovine protein (PDB code 2ACY) (34) with the 3D structures of human protein AcPh-wt (blue) and its designed variant AcPh-des (green). The structures of AcPh-wt and AcPh-des were determined in this work by using multidimentional NMR spectroscopy (see Materials and Methods and SI Appendix for details). (B) Cdc42: structural model of human protein (PDB code 1AN0) as solved by x-ray crystallography in ref. 40.