Table 1.
Effect of ARNT PAS-B mutations on relative populations in conformations
| Mutation | Percentage 1 | Percentage 2 |
|---|---|---|
| Wild type | >99 | <1 |
| F444Q | >99 | <1 |
| F444Q/I454A/Y456T | >98 | <2 |
| F444Q/I454F/Y456T | >98 | <2 |
| F444Q/Y456T/I458E | >98 | <2 |
| Y456A | >98 | <2 |
| Y456S | 81 | 19 |
| Y456T | 50 | 50 |
| P449A/Y456T | 32 | 68 |
| F444Q/T445A/Y456T | 31 | 69 |
| F444Q/Y456T | 19 | 81 |
| F444Q/Y456T/T460V | 15 | 85 |
| F444Q/T445Y/Y456T | 14 | 86 |
| F444Q/N448T/Y456T | 10 | 90 |
| F444Q/N448W/Y456T | <2 | >98 |
| F444Q/Y456T/I457F | <2 | >98 |
| F444Q/F446A/Y456T | <1 | >99 |
For each species, relative populations of conformers 1 and 2 were established by 15N-1H HSQC measurements and comparisons of peak intensities at 10–18 pairs of cross-peaks. Each measurement carries ≈8% relative error on any given ratio.