Fig. 2.

The release of M80A from mitochondria may be peroxidase activity-dependent. (A) Recombinant M80A has higher peroxidase activity than recombinant WT cyt c. The peroxidase activity of recombinant M80A or WT cyt c was determined by luminol oxidation as described previously (32). (B) DEANO, an inhibitor of the peroxidase activity of cyt c (18), inhibits M80A cyt c release from mitochondria. HeLa cells expressing GFP-tagged M80A cyt c were treated in the presence or absence of DEANO (2 mM) for 8 h. Subcellular localization of M80A was then determined by confocal microscopy as described in Fig. 1A. The results are representative of 4 separate experiments.