Fig. 5.

EGFR^vIII signals differently than EGFR^WT. EGF ligand stimulation of EGFR^WT-expressing cells stimulates the activation of mTORC1, whereas mTORC2 appears to be constitutively activated in EGFR^vIII-expressing GBM tumor cells. Western blot analysis of total cell extracts from GBM tumor cells expressing the indicated receptors. Cells were serum starved and stimulated as described previously. Immunoblots were probed with antibodies as indicated.