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. 2009 Feb 5;106(8):2717–2722. doi: 10.1073/pnas.0812304106

Fig. 1.

Fig. 1.

AID expression and RPA interaction in AIDS38A mutant mice. (A) AID protein levels in anti-CD40 plus IL-4–stimulated splenic B cells. Sixty micrograms of whole cell extracts from purified and activated splenic B cells from mice of the indicated genotypes harvested at day 2.5 of stimulation were separated by SDS-PAGE and immunoblotted with antibodies to AID or tubulin. Results from 2 independent experiments (Exp.) are shown. (B) Protein levels in LPS + anti-δ-dextran–treated splenic B cells. Whole cell extracts (60 μg) were analyzed as in A in response to LPS-IgD-dextran stimulation. (C) Interaction of AIDWT and AIDS38A mutant proteins with RPA. Nuclear extracts from AID-deficient (−/−), WT (+/+), and AIDS38/S38A mice were immunoprecipitated (IP) with AID, and the immunoprecipitate was analyzed by Western blotting (WB) for the presence of the 32-kDa subunit of RPA (Top) or AID (Bottom). Ig, Ig chains.