Fig. 3.

IPS-1 overexpression in Atg5^−/− MEFs. (A) Intracellular staining of IPS-1 protein analyzed by FACS in Atg5^+/+ and Atg5^−/− MEFs. (B) Atg5^+/+ and Atg5^−/− MEFs were transduced with lentivirus expressing IPS-1-GFP fusion protein or GFP (control). Cells expressing only high levels of GFP were sorted by FACS and stimulated with 1 μg/mL Poly I:C complexed to Lipofectamine. Twelve hours later, IFN-α and IFN-β mRNA levels were assessed by RT quantitative PCR. *P < 0.05, **P < 0.005. Similar results were obtained from 2 separate experiments.