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. 2009 Feb 5;106(8):2770–2775. doi: 10.1073/pnas.0807694106

Fig. 4.

Fig. 4.

Autophagy-deficient MEFs have increased levels of mitochondrial ROS. Atg5+/+ and Atg5−/− MEFs treated with 1 μM of rotenone for 12 h. (A) Levels of mitochondria associated ROS in Atg5+/+ and Atg5−/− MEFs were analyzed by MitoSOX labeling. (B) After 12 h rotenone pretreatment, cells were transfected with 1 μg/mL Poly I:C. Expression levels of IFN-β were assessed by RT quantitative PCR 12 h after transfection. (C) MEFs were pretreated with 10 mM of the anti-oxidant NAC or 100 μM of PG for 15 min and transfected with Poly I:C (1 μg/mL). IFN-α and IFN-β production was assessed by RT quantitative PCR at 12 h after transfection. *P < 0.05, **P < 0.005. Data are representative of 3 separate experiments.