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. 2009 Feb 6;106(8):2853–2858. doi: 10.1073/pnas.0810558106

Fig. 3.

Fig. 3.

AE-mediated self-renewal is independent on CBFβ expression. (A) RNAs from primary bone marrow cells transduced with virus carrying indicated shRNA were isolated 7 days after transduction and selection, reverse transcribed, and subjected to quantitative PCR. Error bars indicate SD of 2 independent experiments carried out in duplicate. (B) Western blot on primary bone marrow cells transduced with virus carrying indicated shRNAs using α-CBF or α-actin antibodies (for loading control). (C) Bar chart represents the numbers of colonies in each plating of primary hematopoietic cells cotransduced with either AML-ETO or MLL-ENL (control) together with one of the shRNAs as indicated at left. Error bars indicate SD of 6 different experiments. (D) Typical third-round colony morphology of the indicated retroviral-transduced primary bone marrow cells. (E) Immunophenotypic analysis of cells cotransduced by MLL-ENL or AE and indicated shRNAs in the fifth round of plating. Red profiles represent stainings obtained with antibodies specific for the indicated surface markers. Blue profiles show unstained controls. (F) Typical fifth-round cell morphology of primary bone marrow cells transduced with indicated constructs.