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. 2009 Feb 6;106(8):2853–2858. doi: 10.1073/pnas.0810558106

Fig. 5.

Fig. 5.

Rescue of transformation-defective AEΔNHR2 mutant by synthetic homo-oligomerization FKBP modules. (A) Left, Schematic diagram of the constructs used in the gel filtration analysis. Right, Western blot using α-Flag antibody on protein fractions collected from indicated elution volumes, with dotted lines indicating corresponding size standards. Coimmunoprecipitation assay between myc-tagged AE constructs and (B) Flag-tagged ETO family proteins. IP, immunoprecipitation; WB, Western blot. (C) Left, Schematic diagram of AE and the synthetic AML-NHR1-FKBP-NHR3/4 constructs used in RTTA. Right, Bar chart represents the corresponding numbers of colonies in the each round of plating. Error bars indicate SD of 3 independent experiments. (D) Typical third-round colony morphology of the indicated retroviral-transduced primary bone marrow cells. (E) Immunophenotypic analysis of cells transduced by AE or AML1-NHR1-FKBP-NHR3/4. Dot plots represent stainings obtained with antibodies specific for the indicated surface markers. Contour plots indicate unstained controls. (F) Bar chart represents the corresponding numbers of third-round colonies transduced with constructs indicated in the left with or without treatment of AP21998.