Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Peptides. 2008 Jul 4;29(10):1716–1725. doi: 10.1016/j.peptides.2008.06.023

Figure 1.

Figure 1

Two characteristic examples of responses by pre-labeled vagal terminal varicosities to CCK in the perfusion media [A–D represent the analysis of one slice; E–H represent another slice]. The complete field of view of the two brainstem slices in the live cell imaging system are shown in (A) and (E) with the dotted boxes indicating the specific regions of interest [ROI] that were highlighted in the remaining panels. In panels (B) and (F), the ROI is shown immediately before the CCK stimulation. In panels (C) and (G), the ROIs are shown at the peak of their activation response. Panels (D) and (H) are displays, over time, of the change in magnitude of fluorescence [(ΔF/F)%] of the ROI indicted in (C)or (G), respectively, in response to 60sec exposure of the slice to CCK perfusion.

Scale bars: A and E = 80micron; B, C, F, and G = 4micron; D and H = 60sec.