Figure 4. Gli2P1-4 rescues the cell death caused by Shh mutation in the spinal cord.
TUNEL assay was used to detect apoptotic cells in the WT (A), Gli2P1-4/P1-4 (B), Shh−/− (C), and Shh−/−;Gli2P1-4/P1-4 (D) neural tubes of E10.5 embryos. The number of apoptotic cells within the neural tube area is shown by graph in the right (n = 4).