Figure 7. The specification of MNs and V0-V2 interneurons is restored in the Gli2^P1-4;Shh double mutant.

Cross-sections of E10.5 mouse embryos at the forelimb position were stained with antibodies to the proteins shown above the panels. The genotypes are indicated to the left. Note that the FP and V3 interneurons (Foxa2 and Nkx2.2 expressing cells)(A, B, E, and F) are not specified in Gli2^P1-4/P1-4;Shh^−/− embryos. However, MNs (Olig2, Isl, and MNR2 positive cells) and V2-V0 interneurons (Lhx3, En1, and Evx1 positive cells, respectively) are generated, though mispatterned (compare F–H to B–D and I–J to M–N). Similarly, the normal pattern of Pax6 and Pax7 expression is also restored (compare O–P to K–L and Fig. 6).