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. 2009 Mar 5;4(3):e4685. doi: 10.1371/journal.pone.0004685

Figure 5. BP7033Br and BP7033Br ALK inhibited MDA-MB-231 breast cancer cell migration, invasion, MMP-9 and MMP-2 activities.

Figure 5

BPs inhibited MDA-MB-231 breast cancer cell migration (A) and invasion (B). Cells (2.5×105) with 125 µM of BPs were added to each 8 µm-insert in the upper chamber of boyden chamber. After 24 h, cells invading the chamber were fixed, stained and counted as described in “Materials and Methods”. BPs inhibited MMP-9 and MMP-2 activities (C and D, respectively). Lyophilized conditioned media were normalized to the number of cells and subjected to 10% SDS-polyacrylamide gels containing 1 mg/mL gelatine. Lane 1, 2 and 3 represent the control, BP7033Br and BP7033Br ALK conditioned medium of treated cells, respectively. Each column represents a mean (±SD) of three independent experiments. *P versus MDA-MB-231 control<0.05, ** P versus D3H2LN control<0.05.