Figure 2. A ‘division tree’ diagram showing the growth and division of yeast cells from a single original mother cell.
The original mother cell is labelled “1”. Each time a new bud (daughter) appeared on the original mother cell it was labelled 1.1, 1.2, 1.3 etc…and the time of appearance noted. When these daughter cells produced buds they were labelled 1.1.1, 1.1.2 etc… Two sets of data could be derived: a) the successive time taken for a cell to divide multiple times (mother cell reproduction times), and b) the time taken from emergence as a bud on a mother cell to subsequent first division (daughter cell reproduction times).