Skip to main content
. 2009 Feb 14;65(Pt 3):236–241. doi: 10.1107/S1744309109002267

Figure 2.

Figure 2

(a) Size-exclusion chromatography of the N414/439Q mutant protein of mGluR3 ECD. The elution positions of the marker proteins are shown by arrowheads with molecular weights in kDa. The estimated molecular weight is 205 kDa. (b) Chemical cross-linking of the N414/439Q protein. After reaction with EDC, the aliquots were analyzed by SDS–PAGE under reduced conditions. Lane 1, without EDC; lane 2, with EDC for 30 min; lane 3, with EDC for 2 h. The arrowheads indicate the positions of the native/cross-linked proteins.