TABLE 2.
Levels of resistance of S. coelicolor A3(2) mutants to various drugs
| Strain (mutation) | MIC (μg/ml) a
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sm | Par | Km | Neo | FA | Lin | Tet | Gen | G418 | |
| 1147 (wild type) | 1 | 0.1 | 0.8 | 0.3 | 50 | 50 | 10 | 0.1 | 0.3 |
| KO-178 (K88E) | 100 | 0.3 | 0.8 | 0.8 | 50 | 40 | 10 | 0.1 | 0.3 |
| KO-347 (P91S) | 20 | 0.8 | 0.5 | 0.5 | 50 | 50 | 5 | 0.5 | 0.3 |
| 92G (GI92) | 20 | 2 | 1 | 0.5 | 50 | 20 | 10 | 0.5 | 1 |
| SP1 (K88E, P91S) | 200 | 2 | 0.5 | 0.2 | 20 | 40 | 3 | 0.5 | 0.5 |
| SP2 (K88E, GI92) | >200 | 3 | 0.5 | 0.2 | 20 | 10 | 3 | 0.5 | 0.5 |
a
MICs were determined following incubation on GYM plates at 30°C for 48 h. Abbreviations: Sm, streptomycin; Par, paromomycin; Km, kanamycin; Neo, neomycin; FA, fusidic acid; Lin, lincomycin; Tet, tetracycline; Gen, gentamicin; and G418, Geneticin. Boldface indicates a decline in the level of resistance.