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Western blotting with anti-ADC-7 antibody and with protein extracts (10 μg) obtained from the 15 different AG3 isolates indicated above the gel. Purified ADC-7 enzyme (15 ng) (a gift from R. A. Bonomo) (C+) and protein extracts of an Acinetobacter sp. isolate overexpressing the AmpC enzyme (isolate 92) were included as positive controls.
