FIG. 2.
In vitro selection of HIV-1 variants resistant to GRL-02031. HIV-1NL4-3 was passaged in the presence of increasing concentrations of APV (open circles), IDV (solid circles), or GRL-02031 (open triangles) in MT-4 cells. The selection was carried out in a cell-free manner for a total of 21 to 37 passages with drug concentrations escalating from 0.02 to 5 μM. The nucleotide sequences of the proviral DNAs were determined by using cell lysates of HIV-1-infected MT-4 cells at the termination of each indicated passage.