Identification of gB-1 peptides that inhibit HSV-1 infection. A library of 138 overlapping peptides spanning residues 30 to 730 (ectodomain) of gB-1 were synthesized and tested at a concentration of 100 μM in a comprehensive antiviral assay for the ability to inhibit β-galactosidase (β-gal) expression by the hrR3 virus. The top line shows a schematic representation of the gB-1 protein, and the activities of the peptides are shown at the bottom. Arrows denote the seven peptides that were positive in the assay. V, virus-only control; E, inhibition by the EB peptide as a positive control (12); SS, signal sequence; TM, transmembrane domain.