TABLE 1.

Bacterial strains used in study

Strain	Organism	blaTEM or blaSHV derivative	Amino acid substitution(s)a	Source or reference
GC 1472 (R6K)	E. coli	TEM-1	NAb	Wyeth Strain Collection
GC 1494 (CF102)	E. coli	TEM-3	Q-39-K, E-104-K, G-238-S	Wyeth Strain Collection
GC 1500	E. coli	TEM-4	L-21-F, E-104K, G-238-S, T-265-M	Wyeth Strain Collection
GC 1505	E. coli	TEM-7	Q-39-K, R-164-S	Wyeth Strain Collection
GC 1511 (SC15042)	E. coli	TEM-10	R-164-S, E-240-K	Wyeth Strain Collection
GC 2336	E. coli	TEM-28	R-164-H, E-240-K	6
GAR 4649	K. pneumoniae	TEM-29	R-164-H	Wyeth Clinical Studies
GC 2400 (BA156)	E. coli	TEM-43	E-240-K, R-164-H, M-182-T	60
GAR 2830	E. coli	TEM-1	NA	Wyeth Clinical Studies
GAR 3593	P. mirabilis	TEM-1/TEM-2	Q-39-K	Wyeth Clinical Studies
GAR 10941	P. mirabilis	TEM-155	Q-39-K, R-164-S, E-240-K	Wyeth Clinical Studies
GAR 10711	K. pneumoniae	SHV-1	NA	Wyeth Clinical Studies
GAR 11829	K. pneumoniae	SHV-2	G-238-S	Wyeth Clinical Studies
GC 2871 (PAB-C16)	E. coli	SHV-4	R-205-L, G-238-S, E-240-K	7
GAR 9191	K. pneumoniae	SHV-5	G-238-S, E-240-K	Wyeth Clinical Studies
GC 2904 (PAB-C41)	E. coli	SHV-6	D-179-A	7
GC 2337	E. coli	SHV-7	I-8-F, R-43-S, G-238-S, E-240-K	8
GC 5901	E. coli	SHV-8	D-179-N	47 (gift of F. Tenover)
GAR 5951	K. pneumoniae	SHV-11	L-35-Q	Wyeth Clinical Studies
GC 1709 (R1010)c	E. coli	SHV-11	L-35-Q	50 (Wyeth Strain Collection)
GC 6326 (KL-1)	E. coli	SHV-12	L-35-Q, G-238-S, E-240-K	Wyeth Strain Collection (gift of H. Hachler)
GAR 12767	K. pneumoniae	SHV-27	G-156-D	Wyeth Clinical Studies
GC 7991 (A1220)	K. pneumoniae	SHV-1, SHV-5, SHV-105	I-8-F, R-43-S, G-156-D, G-238-S, E-240-K	Wyeth Strain Collection (gift of L. Saiman)
GC 7644 (KP1691)	K. pneumoniae	SHV-1, SHV-5	G-238-S, E-240-K	Wyeth Strain Collection (gift of C. Urban)
GAR 2332	K. pneumoniae	SHV-11, SHV-12	L-35-Q, G-238-S, E-240-K	Wyeth Clinical Studies
GAR 11218	K. pneumoniae	SHV-1, SHV-5, SHV-11, SHV-12	L-35-Q, G-238-S, E-240-K	Wyeth Clinical Studies
GAR 8110	K. pneumoniae	SHV-1, SHV-5, SHV-45	G-156-D, G-238-S, E-240-K	Wyeth Clinical Studies
ATCC 25922	E. coli	None	NA	www.atcc.org
GC 8130d	E. coli DH5α	SHV-1	NA	This study
GC 7668d	E. coli DH5α	SHV-48	I-119-V	This study
GC 8128d	E. coli DH5α	SHV-105	I-8-F, R-43-S, G-156-D, G-238-S, E-240-K	This study
GC 8133d	E. coli DH5α	TEM-155	Q-39-K, R-164-S, E-240-K	This study

^aAmino acid numbering according to Ambler et al. (1).

^bNA, not applicable.

^cStrain originally reported to carry SHV-1.

^dRespective β-lactamase genes shotgun cloned into pCLL2300.