TABLE 2.
Primer | Position of sequence start or size of amplicon | Sequence (5′-3′)a | Comment |
---|---|---|---|
TEM_F1_bio (TEM_F3) | 518 bp | 5′-AGACAATAACCCTGGTAAATGC | PCR primer forward set, 5′ half of the gene, 5′ biotin labeled on forward primer (TEM_F1_bio) |
TEM_R1 (TEM_R3_bio) | 5′-CAAGGCGAGTTACATGATCC | PCR primer reverse set, 5′ biotin on reverse primer (TEM_R3_bio) | |
TEM_F2_bio (TEM_F4) | 460 bp | 5′-ACTTACTTCTGACAACGATCGG | PCR primer forward set, 3′ half of gene, 5′ biotin labeled on forward primer (TEM_F2_bio) |
TEM_R2 (TEM_R4_bio) | 5′-TTACCAATGCTTAATCAGTGAGG | PCR primer reverse set, 5′ biotin on reverse primer (TEM_R4_bio) | |
TEM_dwn_280 | 828, noncoding | 5′-CAGTGAGGCACCTATCTC | Sequencing primer for use with forward set PCR products |
TEM_dwn_165n | 492, noncoding | 5′-GGCTTCATTCAGCTCCGG | Sequencing primer for use with forward set PCR products |
TEM_dwn_104 | 310, noncoding | 5′-GATGCTTTTCTGTGACTGGTG | Sequencing primer for use with forward set PCR products |
TEM_up_265n | 773, coding | 5′-CGTATCGTAGTTATCTACACG | Sequencing primer for use with reverse set PCR products |
TEM_up_238 | 705, coding | 5′-TTGCTGATAAATCTGGAGCC | Sequencing primer for use with reverse set PCR products |
TEM_up_182n | 537, coding | 5′-AACGACGAGCGTGACACCACG | Sequencing primer for use with reverse set PCR products |
TEM_up_69 | 194, coding | 5′-TCGCCCCGAAGAACGTTTTCC | Sequencing primer for use with reverse set PCR products |
TEM_up_39 | 106, coding | 5′-TGAAAGTAAAAGATGCTGAAG | Sequencing primer for use with reverse set PCR products |
TEM_up_21 | 55, coding | 5′-TCCCTTTTTTGCGGCATTTTGC | Sequencing primer for use with reverse set PCR products |
The GenBank accession number for the blaTEM-1 sequence is AY394610.