TABLE 2.

Amplification and pyrosequencing primers for the bla_TEM gene used in the study

Primer	Position of sequence start or size of amplicon	Sequence (5′-3′)a	Comment
TEM_F1_bio (TEM_F3)	518 bp	5′-AGACAATAACCCTGGTAAATGC	PCR primer forward set, 5′ half of the gene, 5′ biotin labeled on forward primer (TEM_F1_bio)
TEM_R1 (TEM_R3_bio)		5′-CAAGGCGAGTTACATGATCC	PCR primer reverse set, 5′ biotin on reverse primer (TEM_R3_bio)
TEM_F2_bio (TEM_F4)	460 bp	5′-ACTTACTTCTGACAACGATCGG	PCR primer forward set, 3′ half of gene, 5′ biotin labeled on forward primer (TEM_F2_bio)
TEM_R2 (TEM_R4_bio)		5′-TTACCAATGCTTAATCAGTGAGG	PCR primer reverse set, 5′ biotin on reverse primer (TEM_R4_bio)
TEM_dwn_280	828, noncoding	5′-CAGTGAGGCACCTATCTC	Sequencing primer for use with forward set PCR products
TEM_dwn_165n	492, noncoding	5′-GGCTTCATTCAGCTCCGG	Sequencing primer for use with forward set PCR products
TEM_dwn_104	310, noncoding	5′-GATGCTTTTCTGTGACTGGTG	Sequencing primer for use with forward set PCR products
TEM_up_265n	773, coding	5′-CGTATCGTAGTTATCTACACG	Sequencing primer for use with reverse set PCR products
TEM_up_238	705, coding	5′-TTGCTGATAAATCTGGAGCC	Sequencing primer for use with reverse set PCR products
TEM_up_182n	537, coding	5′-AACGACGAGCGTGACACCACG	Sequencing primer for use with reverse set PCR products
TEM_up_69	194, coding	5′-TCGCCCCGAAGAACGTTTTCC	Sequencing primer for use with reverse set PCR products
TEM_up_39	106, coding	5′-TGAAAGTAAAAGATGCTGAAG	Sequencing primer for use with reverse set PCR products
TEM_up_21	55, coding	5′-TCCCTTTTTTGCGGCATTTTGC	Sequencing primer for use with reverse set PCR products

^aThe GenBank accession number for the bla_TEM-1 sequence is AY394610.