TABLE 4.

Pyrosequence analysis of bla_TEM enzymes

Strain	β-Lactamase	Amino acid at positiona:
		21	39	69	104	164	182	238	240	265	276
GC 1472	TEM-1	L	Q	M	E	R	M	G	E	T	N
GC 1494	TEM-3	L	K	M	K	R	M	S	E	T	N
GC 1500	TEM-4	F	Q	M	K	R	M	S	E	M	N
GC 1505	TEM-7	L	K	M	E	S	M	G	E	T	N
GC 1511	TEM-10	L	Q	M	E	S	M	G	K	T	N
GC 2336	TEM-28	L	Q	M	E	H	M	G	K	T	N
GC 2400	TEM-43	L	Q	M	K	H	T	G	E	T	N
GAR 2830	TEM-1	L	Q	M	E	R	M	G	E	T	N
GAR 3593	TEM-1 (50),b TEM-2 (50)	L	Q/K	M	E	R	M	G	E	T	N
GAR 4649	TEM-29	L	Q	M	E	H	M	G	E	T	N
GAR 10941	TEM-155	L	K	M	E	S	M	G	K	T	N

^aAmino acid numbering according to Ambler et al. (1). Boldface and underlining indicate amino acid residue substitutions identified by pyrosequence analysis.

^bValues in parentheses are the percentage of bla_TEM alleles determined on the basis of the percentage of each SNP at amino acid 39 codon (Fig. 2).