FIG. 3.

Clearance and rebound experiment with Huh 9-13 cells. (A) Cells were treated for seven consecutive passages with either 1 μg/ml (0.82 μM) Debio 025 (red), 5 μg/ml (7.06 μM) VX-950 (blue), 30 μg/ml (122.85 μM) RBV (green), or 100 IU/ml IFN (black) in the absence of selective G418 pressure. The HCV RNA content was quantified at every passage by means of RT-qPCR and is expressed as the percentage of the HCV RNA content of the untreated (control) sample at the same passage number. (B) During rebound, the compounds were omitted from the culture medium but the cells were again cultured under the selective pressure of G418 (1,000 μg/ml). Following every passage, two rebounds were inserted; on the x axis, the first number refers to the passage number, while the second number stands for the rebound number. Again, the HCV RNA content was quantified and expressed as a percentage of the HCV RNA content of the untreated (control) sample at the same passage and rebound number. (C) Cells were treated for four consecutive passages with 1 μg/ml (0.82 μM) Debio 025, 30 μg/ml (122.85 μM) RBV, and 100 IU/ml IFN in the absence of selective G418 pressure. During rebound, the compounds were omitted from the culture medium but the cells were again cultured under the selective pressure of G418 (1,000 μg/ml). Following every passage, two rebounds were inserted. P, passage; R1, rebound 1; R2, rebound 2.