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Plaque size reduction assays. Confluent monolayers of BSC-40 cells were infected with 25 PFU of VARV (top panel) or MPXV (bottom panel). Infected cells were incubated with various concentrations of ST-246 (none, 0.5, 0.15, 0.05, 0.015, or 0.005 μM) in RPMI (2 ml) containing 2% FBS and 1% CMC. Plaque sizes were visualized by IHC staining.
