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. 2008 Dec 15;53(3):1107–1115. doi: 10.1128/AAC.01107-08

TABLE 3.

Oligonucleotides relevant for this study

Oligonucleotide and use	Sequence (5′→3′)
For site-directed mutagenesis
pbp2x_5	GATTGCTGAGGATGCAACCTCTTATAATGTCTATG
pbp2x_3	GCCTTGAAATTCAAGTTCTATATTGAGCCACTTAGC
pbp2x_A-FM^a	ATCATCACTTTCATGGCGGAACCTGGCTCATAGTTAC
pbp2x_A-RM^a	TATGAGCCAGGTTCCGCCATGAAAGTGATGATGTTGG
pbp2x_P-FM^a	TCATCACTTTCATAGGGGAACCTGGCTCATAGTTAC
pbp2x_P-RM^a	AACTATGAGCCAGGTTCCCCTATGAAAGTGATGATG
pbp2x_G-FM^a	ATCATCACTTTCATGCCGGAACCTGGCTCATAGTTAC
pbp2x_G-RM^a	TATGAGCCAGGTTCCGGCATGAAAGTGATGATGTTG
For cia and pbp1a derivatives
CiaR_for	TTGGCAGCAGCTCTTGCATACAGTACAG
CiaR_rev	ACCGAAATCGTTGTATCACTATCAAATCC
Ja-pbp1a_for	GGCTGGTGCCTTCCCTCAAATTCCTTATC
Ja-pbp1a_rev	CAAGTGTTAGCCTAGCCCTATCTGCTCTC
Ja-pbp1a-seq_for^b	CATAAGGAAAGGGGCCCTAGAACCGCGACTGGGGATCAACTATG
Ja-pbp1a-seq_rev^b	CCATTTCCTCTGGAATAGGCTGTTTCTACTGCTTGGTTAATTCC
Ja-Kass_for^b	TTAACCAAGCAGTAGAAACAGCCTATTCCAGAGGAAATG
Ja-Kass_rev^b	GTTGATCCCCAGTCGCGGTTCTAGGGCCCCTTTCCTTATGC

^a

The mutated codon is underlined.

^b

The overlapping termini are shown in italics.