Polyacrylamide gel analysis of gap-LCR assay. Lane 1, low-molecular-weight DNA ladder (New England Biolabs, Beverly, MA); lane 2, pBAD24-aac(6′)-Ib-cr; lane 3, pBAD24-aac(6′)-Ib; lane 4, aac(6′)-Ib-cr-negative E. coli; lane 5, aac(6′)-Ib-cr-positive E. coli. Arrow points to the putative reaction product.