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. 2009 Jan 5;53(3):1039–1047. doi: 10.1128/AAC.01446-08

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Copyright © 2009, American Society for Microbiology

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FIG. 1. — Detection of established molecular markers of resistance to M2 blockers in H5N1 viruses using pyrosequencing. The sequences generated encompass the region of the M gene which contains the 5-amino-acid residues of M2 protein associated with resistance. (A) A/Hong Kong/482/1997 (sensitive) has no change at any of the 5 codons (blue). (B) A/chicken/Vietnam/NCVD-093/2008 (resistant) contains the mutation S31N (AGT to AAT). (C) A/Vietnam/1203/2004 (resistant) contains two mutations, L26I (CTT to ATT) and S31N (AGT to AAT). (D) A/duck/Vietnam/NCVD-19/2007 (resistant) contains three mutations, L26I (CTT to ATT), V27A (GTT to GCT), and S31N (AGT to AAT).