Figure 3.

Identification of Revolver mRNA. (A) Multiple sizes of transcripts homologous to Revolver were found in rye but no transcript was found in wheat. (B) A full-length Revolver cDNA clone (AB124665–124666), 726 bp long, was identified. Nucleotide sequence comparison between Revolver and the cDNA revealed that Revolver includes a single gene consisting of three exons and two introns. The predicted 139 amino acid sequence is highly conserved in Revolver cDNAs (AB124645, AB124666, AB304271–304275), which are recovered from S. cereale, S. silvestre, D. villosum, and Ae. tauschii, indicating activity in all of these species. The ORF shows similarity to some DNA-binding proteins. The Revolver ORF includes a DDE motif. The initial 89 bp clone (AB304276) obtained by genomic subtraction corresponds to the second exon. Revolver (AB124639, AB124640) shows 60% homology to parts of both ends (5′ side 104 bp, 3′ side 2213 bp) of LARD (4327 bp), however, in which the region extending from exon 1 to the half of intron 1 of Revolver is replaced by a different sequence and the coding region is not present.