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. 2009 Feb 25;8:10. doi: 10.1186/1476-4598-8-10

Figure 2.

Figure 2

Depletion of NEDD1 reduces cell proliferation of HCT116 p53+/+ and HCT116 p53 -/- cells. (A) Immunoblots of HCT116 p53+/+ and HCT116 p53 -/- cells treated with control siRNA or siRNA targeting NEDD1, at 50 nM for 72 h. Blots were probed with antibodies against NEDD1 and actin. (B) Flow-cytometric analysis of HCT116 p53+/+ and HCT116 p53 -/- cells treated with control RNA or NEDD1-siRNA for 48 h, and labelled with propidium iodide (C) Number of living cells quantified by trypan blue exclusion analysis, 48 h after transfection of control RNA or NEDD1-siRNA, at 25 nM (error bars represent SD from three independent experiments).