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. 2009 Feb 25;9:44. doi: 10.1186/1471-2180-9-44

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Copyright ©2009 Cecere and Cogoni; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bidirectional transcription from NTS rDNA locus. Radioactive RT-PCR analysis to detect transcripts derived from NTS rDNA region. Reverse transcription was carried out with specific oligos for NTS rDNA and actin as control and show a signal of the right size from forward and reverse strand of NTS rDNA locus compared to the reaction without reverse transcriptase enzymes. * indicate a signal from genomic rDNA locus (more abundant then actin locus), but that is weak compared to the RT+ lane and therefore reflects the presence of NTS transcripts.