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. 2000 Feb 4;97(4):1749–1753. doi: 10.1073/pnas.040560897

Figure 2.

Figure 2

Constitutively active forms of PI 3-kinase and Akt induce multiple abnormally large capillaries and proliferation of endothelial cells. The CAMs infected with virus as above were snap frozen, and 6-μm cryostat sections of CAMs were fixed in 3.7% formaldehyde and stained by immunofluorescence with factor VIII antibodies (BioGenex). (A) Representative fields showing CAMs 7 days after infection by RCAS, RCAS-v-P3k, and RCAS-Myr-Akt virus (×40 objective). (Upper) Immunofluorescence with factor VIII antibodies. (Lower) Phase-contrast. (B) Representative fields were stained with factor VIII antibodies, showing increased numbers of endothelial cells that form large luminal structures 9 days after infection.