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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: DNA Repair (Amst). 2008 Dec 9;8(2):279–284. doi: 10.1016/j.dnarep.2008.10.012

Figure 2. Dissociation of the SpDmc1-dsDNA complex by SpRdh54.

Figure 2

(A) The reaction schematic is shown.

(B) Nucleoprotein complexes of SpDmc1 and magnetic bead-bound DNA were assembled with either ATP or AMP-PNP as nucleotide cofactor and then incubated without or with SpRdh54 (200 nM in lane 3, 400 nM in lanes 4 and 8, 600 nM in lane 5, and 750 nM in lanes 6 and 9). The supernatant and bead fractions were analyzed by SDS-PAGE and Coomassie Blue staining for their protein content. CK, creatine kinase used in the ATP regenerating system. The results were plotted.