(A) The reaction schematic is shown.
(B) Nucleoprotein complexes of SpDmc1 and magnetic bead-bound DNA were assembled with either ATP or AMP-PNP as nucleotide cofactor and then incubated without or with SpRdh54 (200 nM in lane 3, 400 nM in lanes 4 and 8, 600 nM in lane 5, and 750 nM in lanes 6 and 9). The supernatant and bead fractions were analyzed by SDS-PAGE and Coomassie Blue staining for their protein content. CK, creatine kinase used in the ATP regenerating system. The results were plotted.