Figure 1. Loss of CeRictor Causes Increased Lipid Storage and Decreased Body Size.

(A) Images of Nile Red staining of lipids in anterior intestinal cells of adult wild-type, lpo-6 (mg360), and lpo-6 (ft7) strains. Representative images are shown. In each image, anterior of the animal is to the right.

(B) Quantification of Nile Red staining. Mean fluorescence intensity is reported as a percentage of the mean for wild-type animals (error bars indicate s.e.m.). Single asterisk indicates a p-value < 0.05, and double asterisk indicates p-value <0.01 (wild type versus mutant, two-tailed t-test, n = 5–7).

(C) Quantification of Nile Red staining. Mean fluorescence intensity is reported as a percentage of the mean for wild-type animals (error bars indicate s.e.m.). Single asterisk indicates a p-value ≤ 0.05, and double asterisk indicates p-value < 0.01 (wild type versus mutant, two-tailed t-test, n = 5–8). lpo-6 (+) and (-) refer to the presence or absence of wild-type CeRictor transgene.

(D) Quantification of body size measurements. The perimeter of each worm was measured. Values reported as mean size as a percentage of mean for wild type (error bars indicate s.e.m.). Double asterisk indicates p-value < 0.01 (wild type versus mutant strain, two-tailed t-test, n = 10).

(E) Quantification of body size measurements. The perimeter of each worm was measured. Values reported as mean size as a percentage of mean for wild type (error bars indicate s.e.m.). Double asterisk indicates p-value < 0.01 (wild type versus mutant strain, two-tailed t-test, n = 10). lpo-6 (+) and (-) refer to the presence or absence of wild-type CeRictor transgene. Body size of rescued lpo-6 mutants (lpo-6 (mg360); lpo-6 (+)) is not significantly different from wild-type animals (p-value = 0.88, two-tailed t-test, n = 10).