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. 2009 Mar 3;7(3):e1000060. doi: 10.1371/journal.pbio.1000060

Figure 5. CeRictor and sgk-1 Define a Single Regulatory Pathway Governing Fat Storage, Body Size, and Developmental Rate.

Figure 5

(A) Images of Nile Red staining of lipids in anterior intestinal cells.

(B) Quantification of Nile Red staining. Mean fluorescence intensity is reported as a percentage of the mean for wild-type animals (error bars indicate s.e.m.). Single asterisk indicates p-value < 0.05, and double asterisk indicates p-value < 0.01 (wild type versus mutant, two-tailed t-test, n = 5–6). lpo-6 (ft7) is not significantly different from sgk-1 (two-tailed t-test, p-value = 0.20). sgk-1 does not statistically differ from sgk-1;lpo-6 (ft7) or sgk-1;daf-16 (two-tailed t-tests, p-values = 0.63 and 0.81, respectively).

(C) Quantification of body size. Values reported as mean size as a percentage of mean for wild type (error bars indicate s.e.m.). Double asterisk indicates p-value < 0.01 (wild type versus mutant strain, two-tailed t-test, n = 10). sgk-1 does not statistically differ from sgk-1;lpo-6 (ft7) or sgk-1;daf-16 (two-tailed t-tests, p-values = 0.11 and 0.14, respectively).

(D) Developmental timing of mutant strains. Animals were grown from synchronized L1s for 72 h at 20 °C; individuals were then scored for developmental stage. Sixty–185 animals were used for each strain.