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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Cancer Res. 2009 Feb 17;69(5):1792–1799. doi: 10.1158/0008-5472.CAN-08-2304

Fig. 2. Overexpression of DEK protein in normal immortalized keratinocytes stimulates transformation in vitro.

Fig. 2

A. Western blot analyses. NIKs were transduced with either R780 or R780-DEK and sorted for GFP expression. Sorted NIKs were subsequently transduced with oncogenic hRas-V12 and either HPV E7 alone or HPV E6 and E7 in combination. Total protein lysates were subjected to DEK, cyclin A, p53, p21, PCNA and actin specific western blot analyses. B. Soft agar colony assay. Cells from (A) were submerged in 0.4% agarose, overlaid on top of a 0.8% agarose underlay and monitored for colony formation over three weeks, at which point colonies were counted. Two asterisks indicate a p value of less than 0.01.