Figure 9. Analysis of cell cycle upon rapamycin treatment.

Normal and transformed cells, synchronized by 24 hours of serum starvation, were released in a medium containing 10% serum, 4 mM glutamine±Rapamycin or 0 mM glutamine±Rapamycin and±dNTPs and BrdU for continuous DNA labeling. At indicate time points the cells were collected and stained with propidium iodide and anti-BrdU specific antibody and analyzed by flow cytometry. The percentages of cells in G₁ (panels A and B), S (panels C and D) and G₂/M (panels E and F) phase of normal (left panels) and transformed (right panels) cells, released in the mediums indicated above, and obtained by bi-parametric analysis are represented. The asterisks indicate the S or G₂/M phase peaks identified in both glutamine availability. The error bars correspond to standard deviations of triplicate analysis.