Table 3. Inter-day variations in PVS-RIPO-mediated CPE on U87-MG and HEK293 cells.

Exp#	U87-MG								HEK293
	Cell Passage #	A*	B	C (MOI)	D	R2	ED50 (MOI)	MTS50	Effect	Cell Passage #
1	3	1.546	0.587	0.031	0.434	0.991	0.117	4.07	no effect <1MOI	3
2	4	1.453	0.670	0.038	0.255	0.996	0.070	4.30	no effect <1MOI	4
3	31	1.760	0.848	0.047	0.073	0.990	0.054	4.42	no effect <1MOI	14
4	7	1.486	0.761	0.014	0.413	0.993	0.042	4.50	no effect <1MOI	7
5	10	1.237	0.919	0.022	0.470	0.987	0.094	4.17	no effect <1MOI	10
6	5	1.515	0.478	0.009	0.386	0.994	0.037	4.54	no effect <1MOI	4
7	5	1.334	0.718	0.015	0.240	0.996	0.027	4.72	no effect <1MOI	4
8	3	2.225	0.510	0.003	0.562	0.986	0.010	5.17	no effect <1MOI	3
Average		1.570	0.686	0.022	0.354	0.992	0.056	4.49	no effect <1MOI
Std. Dev.		0.286	0.146	0.014	0.146	0.004	0.033	0.32	-
CV%		18.25	21.33	63.28	41.09	0.36	59.09	7.21	-

^*A, B, C and D are the curve-fit parameters, R² is the curve fit correlation.

ED₅₀ refers to the virus infectious dose (MOI) that causes 50% cell killing.

C value refers to the virus infectious dose (MOI) that causes half maximal cell killing

MTS₅₀ refers to the 50% infectious dose (log₁₀ dilution factor/mL) at which the average infected well absorbance is 50% of the average uninfected cell absorbance