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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Infect Dis. 2009 Mar 1;199(5):684–692. doi: 10.1086/596656

Table 2.

Oligonucleotides Used in This Study

Uses Forward Primera Forward Primera
recombinant expression
of fgbA (HD0192) 		CCTCAAGTTGAAGAAATGAAACA
AACGG		 CGAATTCGTATTTGGTAATAA
ATGACCGC
recombinant expression
of HD0581 		TGTAATAAACCTGATCCTGCTACA
GA		 AGTCATTTGAAAGTCCTATGTC
AG
recombinant expression
of HD1218 		ACCATCTCTGCACACGCAACCATA GGGACCATTCCTAATATGCAA
ATCC
mutagenesis of fgbA catatcggatccCCGCCAACGTTTAAGC
CCATCATT		 catatctctagaTCGGGCGACTTTAG
CGCAATATCA
trans–complementation
with fgbA;
survey of fgbA loci in H.
ducreyi strains		 taagaatgcggccgcTACGCTGCGCCACA
GACTACTAAA		 taagaatgcggccgcTGACCGCGATA
AGCGGTCTTT
class I-specific primers dsrA 14 and
dsrA 24b 		GACAGCATTCAGTGAATAATGGC AATGAAGTCCGCACCTTTAAC
GGC
class II-specific primers
dsrA 42 and dsrA 43c 		TGCCTTGCTCTTAATGACG TAAAAGCACATAAACAAGCG
a

Lowercase lettering indicates linkers with non-H.ducreyi sequences

b

Primers were exactly the same as the class I-specific primer pair of White et al. [24].

c

Primers were the H. ducreyi-specific portions, excluding linker sequences, of the class II-specific primer pair of White et al. [24].