Table 2.
Uses | Forward Primera | Forward Primera |
---|---|---|
recombinant expression of fgbA (HD0192) |
CCTCAAGTTGAAGAAATGAAACA AACGG |
CGAATTCGTATTTGGTAATAA ATGACCGC |
recombinant expression of HD0581 |
TGTAATAAACCTGATCCTGCTACA GA |
AGTCATTTGAAAGTCCTATGTC AG |
recombinant expression of HD1218 |
ACCATCTCTGCACACGCAACCATA | GGGACCATTCCTAATATGCAA ATCC |
mutagenesis of fgbA | catatcggatccCCGCCAACGTTTAAGC CCATCATT |
catatctctagaTCGGGCGACTTTAG CGCAATATCA |
trans–complementation with fgbA; survey of fgbA loci in H. ducreyi strains |
taagaatgcggccgcTACGCTGCGCCACA GACTACTAAA |
taagaatgcggccgcTGACCGCGATA AGCGGTCTTT |
class I-specific primers dsrA 14 and dsrA 24b |
GACAGCATTCAGTGAATAATGGC | AATGAAGTCCGCACCTTTAAC GGC |
class II-specific primers dsrA 42 and dsrA 43c |
TGCCTTGCTCTTAATGACG | TAAAAGCACATAAACAAGCG |