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Effect of mutations in the algD operon on the production of alginate polymer and alginate fragments
| Strain | Mutation | Alginate (mucoid) | Alginate fragments (μg ml−1)* |
|---|---|---|---|
| FRD1 | Wild-type | + | 0 |
| FRD1200 | ΔalgG | − | 1200 |
| FRD1108 | Δalg8 | − | 0 |
| FRD1144 | alg44 | − | 0 |
Secretion of alginate fragments was determined as previously described (Jain & Ohman, 2005) by the demonstration of uronic acids in culture supernatants of non-mucoid strains that are lost by exhaustive dialysis.
