Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Dev Biol. 2008 Oct 17;325(1):82–93. doi: 10.1016/j.ydbio.2008.09.031

Figure 2. Targeted disruption of plexinD1.

Figure 2

(A) A schematic illustration of the plexinD1 locus, targeting vector, and the targeted locus after Flp-mediated recombination. (B) The 5′ external probe as shown in (A) was used for Southern blot analysis to detect heterozygous recombinants using BglII-digested genomic DNA. (C) PCR of tail DNA from plexinD1flox/+ mice confirms germline transmission. The 580bp band represents the wild-type allele, while the 620bp band indicates the targeted allele. (D–I) Absence of plexinD1 mRNA in the vascular endothelium of E12.5 D1ECKO embryos (G,H,I) is demonstrated by in situ hybridization, while plexinD1 transcripts are detected in other tissues including trigeminal ganglion (arrow) and the smooth muscle of the small intestine (double arrow). The D1ECKO embryo has persistent truncus arteriosus (D). A, aorta. P, pulmonary artery. TA, truncus arteriosus.