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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Dev Biol. 2008 Oct 17;325(1):82–93. doi: 10.1016/j.ydbio.2008.09.031

Figure 6. PlexinD1 functions in postnatal angiogenesis.

Figure 6

(A) Quantitative PCR of retina shows effective deletion of plexinD1 after tamoxifen treatment in D1CMV-ERKO mice compared to control (CTRL). The relative expression of PlexinD1 is 0.08±0.04 fold of the control. (n=3) (B) Confocal images pseudocolored to indicate depth of vascular structures in retinal flatmounts from a control P10 pup reveals 2 layers of retinal vessels (green = top layer, 24 micron depth; red = bottom layer, 28.5 micron depth). (C) Confocal image of a D1CMV-ERKO P10 pup shows only one layer of vessels on the surface of retina with fewer deep vessels. (D–F) FITC-dextran perfusion of control (D) and mutant (E,F) retina samples reveals vascular leakage in mutants both from large vessels (E) and from smaller vascular plexi at the periphery of the retina (F). scale bar equals to 50 μm. (G–I) Confocal images of control (G) and mutant (H,I) retina flat mounts show hemorrhage on the retina surface (H), and in the deep layer (I) of mutants.