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. Author manuscript; available in PMC: 2010 Jan 22.
Published in final edited form as: J Med Chem. 2009 Jan 22;52(2):247–258. doi: 10.1021/jm800174p

Figure 5. Effects of bivalent ligands on bystander BRET.

Figure 5

Shown are the saturation curves obtained by co-expressing pairs of Rlu-tagged MOP receptor and YFP-tagged CCK2 receptor constructs after incubation with bivalent ligands (10−6M), as indicated. Bivalent ligands 3a-c produced exponential curves that reached a plateau, supporting specific molecular interactions. Cells treated with either no ligand or with a combination of monovalent ligands for the CCK and mu-opioid receptors showed curves not significantly different from linear fits. Values represent means±S.E.M of data from five independent experiments.