FIG. 1.

(A) Confocal spinning-disk microscopy images and flow cytometry analysis of fixed, Alexa Fluor 488-conjugated bacterial particles coincubated with C2BBe monolayers 6 days after cells were seeded onto permeable filter supports (green, bacterial particles; red, ZO-1; gray, DAPI). Flow cytometry gates indicate cells positive for uptake of fluorescently labeled bacteria. (B) Intestinal and bronchoepithelial cells cultured in the presence of LTβR agonist and TNF-α ingest more S. aureus and Y. enterocolitica particles than do untreated monolayers.