FIG. 3.

NCI-H292 endocytosis of S. aureus at tenfold increases of particle/cell ratios in untreated and TNF/LT-treated cell layers analyzed by flow cytometry (A) and TEM (B). The arrow separates a population of cells which ingested very high (≥100/cell) numbers of bacteria at high bacterial load; load ratios greater than 200/cell increased lysis/cell death. Flow cytometry data are representative of three independent experiments. The cell depicted in panel B is representative of those observed by TEM in cytokine-treated cultures at a bacterial particle/cell ratio of 200:1, and has ingested >100 Staphylococcus particles.