FIG. 5.

Effect of blocking β1 integrin receptors on bacterial particle uptake in airway (A) and intestinal (B) epithelial cells. β1 blockade reduced uptake of S. aureus in untreated and cytokine-treated H292 cells by averages of 5.0 and 18.9%, respectively, with the most noticeable reduction in the population of cells which by fluorescence intensity and side scatter were shown to have taken up >100 particles. Uptake of Yersinia was similarly reduced, although the effect was more pronounced (12.5% reduction) in airway cells treated with TNF/LT. S. aureus uptake was not notably affected by blocking β1 integrin in C2BBe cells; blockade reduced uptake by 4.5 and 2.8% in untreated and treated cells, respectively. All data are means from at least three independent experiments.