TABLE 2.
Detection by ENIA of PA in plasma from mice injected with different doses of toxina
Sample no. | PA dose injected (μg) | PA concn detected (μg/ml) |
---|---|---|
2 | 100 | 26.80 ± 5.9 |
8 | 50 | 19.00 ± 3.4 |
6 | 10 | 1.60 ± 0.8 |
5 | 5 | 0.24 ± 0.1 |
4 | 1 | 0.08 ± 0.01 |
7 | 0 | 0.00 |
Mice (n = 1 per dose) were injected with uncleavable PA (PA-U7) and were bled 2 h after injection. PA levels were then assessed by ENIA. The samples were diluted from 1:10 to 1:1,000.